Hugo_Symbol Entrez_Gene_Id Center NCBI_Build Chromosome Start_position End_position Strand Variant_Classification Variant_Type Reference_Allele Tumor_Seq_Allele1 Tumor_Seq_Allele2 dbSNP_RS dbSNP_Val_Status Tumor_Sample_Barcode Matched_Norm_Sample_Barcode Match_Norm_Seq_Allele1 Match_Norm_Seq_Allele2 Tumor_Validation_Allele1 Tumor_Validation_Allele2 Match_Norm_Validation_Allele1 Match_Norm_Validation_Allele2 Verification_Status Validation_Status Mutation_Status Sequencing_Phase Sequence_Source Validation_Method Score BAM_file Sequencer Tumor_Sample_UUID Matched_Norm_Sample_UUID Genome_Change Annotation_Transcript Transcript_Strand Transcript_Exon Transcript_Position cDNA_Change Codon_Change Protein_Change Other_Transcripts Refseq_mRNA_Id Refseq_prot_Id SwissProt_acc_Id SwissProt_entry_Id Description UniProt_AApos UniProt_Region UniProt_Site UniProt_Natural_Variations UniProt_Experimental_Info GO_Biological_Process GO_Cellular_Component GO_Molecular_Function COSMIC_overlapping_mutations COSMIC_fusion_genes COSMIC_tissue_types_affected COSMIC_total_alterations_in_gene Tumorscape_Amplification_Peaks Tumorscape_Deletion_Peaks TCGAscape_Amplification_Peaks TCGAscape_Deletion_Peaks DrugBank ref_context gc_content CCLE_ONCOMAP_overlapping_mutations CCLE_ONCOMAP_total_mutations_in_gene CGC_Mutation_Type CGC_Translocation_Partner CGC_Tumor_Types_Somatic CGC_Tumor_Types_Germline CGC_Other_Diseases DNARepairGenes_Role FamilialCancerDatabase_Syndromes MUTSIG_Published_Results OREGANNO_ID OREGANNO_Values t_alt_count t_ref_count validation_alt_allele validation_method validation_status validation_tumor_sample pox qox pox_cutoff isArtifactMode oxoGCut UBC 7316 broad.mit.edu 37 12 125397369 125397369 + Missense_Mutation SNP T T C TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr12:125397369T>C uc001ugs.4 - 1 1407 c.949A>G c.(949-951)Atc>Gtc p.I317V UBC_uc001ugr.3_5'Flank|UBC_uc001ugt.3_Missense_Mutation_p.I317V|UBC_uc001ugu.1_Missense_Mutation_p.I317V|UBC_uc001ugv.3_Intron|UBC_uc021rge.1_Missense_Mutation_p.I317V|UBC_uc001ugw.3_Missense_Mutation_p.I165V NM_021009 NP_066289 P0CG48 UBC_HUMAN Homo sapiens ubiquitin C (UBC), mRNA. 317 Ubiquitin-like 5. DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest|DNA repair|G1/S transition of mitotic cell cycle|I-kappaB kinase/NF-kappaB cascade|JNK cascade|M/G1 transition of mitotic cell cycle|MyD88-dependent toll-like receptor signaling pathway|MyD88-independent toll-like receptor signaling pathway|S phase of mitotic cell cycle|T cell receptor signaling pathway|Toll signaling pathway|activation of MAPK activity|anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process|anti-apoptosis|apoptosis|cellular membrane organization|endosome transport|epidermal growth factor receptor signaling pathway|induction of apoptosis by extracellular signals|innate immune response|mRNA metabolic process|negative regulation of epidermal growth factor receptor signaling pathway|negative regulation of type I interferon production|negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle|nerve growth factor receptor signaling pathway|positive regulation of I-kappaB kinase/NF-kappaB cascade|positive regulation of NF-kappaB transcription factor activity|positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle|stress-activated MAPK cascade|toll-like receptor 1 signaling pathway|toll-like receptor 2 signaling pathway|toll-like receptor 3 signaling pathway|toll-like receptor 4 signaling pathway|viral reproduction cytosol|endocytic vesicle membrane|endosome membrane|nucleoplasm|plasma membrane protein binding breast(3)|endometrium(4)|kidney(4)|large_intestine(2)|lung(13)|ovary(3)|prostate(4)|skin(1)|upper_aerodigestive_tract(1)|urinary_tract(1) 36 all_neural(191;0.101)|Medulloblastoma(191;0.163) OV - Ovarian serous cystadenocarcinoma(86;6.17e-05)|Epithelial(86;0.000207)|all cancers(50;0.00308) TCGAGAGTGATGGTCTTACCA 0.522000 40 116 0 0 1 0 0 MLIP 90523 broad.mit.edu 37 6 53986246 53986246 + Splice_Site SNP T T C TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr6:53986246T>C uc011dxa.2 + 2 130 c.97_splice c.e2-1 p.V33_splice MLIP_uc003pcf.2_Splice_Site_p.V22_splice|MLIP_uc003pcg.4_Splice_Site_p.V22_splice|MLIP_uc003pch.4_Intron|MLIP_uc011dwz.1_Intron NM_138569 NP_612636 Q5VWP3 MLIP_HUMAN Homo sapiens muscular LMNA-interacting protein (MLIP), mRNA. 22 Interaction with LMNA. PML body|nuclear envelope protein binding endometrium(1)|haematopoietic_and_lymphoid_tissue(1)|kidney(1)|large_intestine(1)|lung(16)|ovary(8)|skin(4)|stomach(1)|urinary_tract(1) 34 GTCTCATAGGTCTCTGCTGGT 0.393000 20 41 0 0 1 0 0 KIAA1751 85452 broad.mit.edu 37 1 1887133 1887133 + Missense_Mutation SNP C C T TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr1:1887133C>T uc009vkz.1 - 16 2192 c.2173G>A c.(2173-2175)Gca>Aca p.A725T KIAA1751_uc001aim.1_Missense_Mutation_p.A725T NM_001080484 NP_001073953 Q9C0B2 K1751_HUMAN Homo sapiens KIAA1751 (KIAA1751), mRNA. 725 breast(1)|endometrium(5)|kidney(4)|large_intestine(6)|lung(12)|ovary(2)|pancreas(1)|upper_aerodigestive_tract(1) 32 all_cancers(77;0.000708)|all_epithelial(69;0.000943)|all_lung(157;0.00963)|Lung NSC(156;0.0232)|Ovarian(185;0.0634) all_epithelial(116;6.04e-15)|all_lung(118;9.67e-07)|Lung NSC(185;5.59e-05)|Renal(390;0.00571)|Breast(487;0.0183)|Hepatocellular(190;0.0268)|Myeloproliferative disorder(586;0.028)|Ovarian(437;0.127)|Medulloblastoma(700;0.151)|Lung SC(97;0.217) Epithelial(90;8.79e-39)|OV - Ovarian serous cystadenocarcinoma(86;9.61e-25)|GBM - Glioblastoma multiforme(42;1.2e-07)|Colorectal(212;4.84e-05)|COAD - Colon adenocarcinoma(227;0.000214)|Kidney(185;0.00254)|BRCA - Breast invasive adenocarcinoma(365;0.00461)|STAD - Stomach adenocarcinoma(132;0.00645)|KIRC - Kidney renal clear cell carcinoma(229;0.037)|Lung(427;0.205) GGCTCACCTGCGGGCTGCTCC 0.692000 13 26 0 0 1 0 0 TBC1D2B 23102 broad.mit.edu 37 15 78305349 78305349 + Silent SNP G G A TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr15:78305349G>A uc002bcy.4 - 8 2086 c.2086C>T c.(2086-2088)Ctg>Ttg p.L696L TBC1D2B_uc010bla.3_Silent_p.L696L|TBC1D2B_uc002bda.3_Silent_p.L148L NM_144572 NP_653173 Q9UPU7 TBD2B_HUMAN Homo sapiens TBC1 domain family, member 2B (TBC1D2B), transcript variant 1, mRNA. 696 Rab-GAP TBC. intracellular Rab GTPase activator activity|protein binding breast(3)|cervix(2)|endometrium(4)|kidney(1)|large_intestine(7)|lung(2)|ovary(1)|prostate(2)|skin(2)|upper_aerodigestive_tract(2) 26 GCCTTCTGCAGCAAGGTCTGG 0.562000 3 20 0 0 1 0 0 PDE4D 5144 broad.mit.edu 37 5 58511659 58511659 + Silent SNP G G A TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr5:58511659G>A uc003jsa.2 - 1 763 c.591C>T c.(589-591)agC>agT p.S197S PDE4D_uc003jrx.2_Silent_p.S61S|PDE4D_uc003jry.3_5'UTR|PDE4D_uc003jrz.3_Silent_p.S133S|PDE4D_uc003jsb.3_Silent_p.S136S|PDE4D_uc003jsc.3_Silent_p.S133S|PDE4D_uc003jrv.2_Silent_p.S67S|PDE4D_uc003jrw.2_Silent_p.S75S|PDE4D_uc010iwi.1_Silent_p.S29S NM_001104631 NP_001098101 Q08499 PDE4D_HUMAN Homo sapiens phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 1, mRNA. 197 signal transduction cytosol|insoluble fraction|membrane|microtubule organizing center|soluble fraction 3',5'-cyclic-AMP phosphodiesterase activity|metal ion binding breast(1)|central_nervous_system(1)|endometrium(1)|kidney(1)|large_intestine(2)|lung(8)|pancreas(1) 15 all_cancers(5;6.5e-58)|all_epithelial(5;1.75e-57)|all_lung(5;6.84e-18)|Lung NSC(5;1.29e-17)|Melanoma(5;0.00168)|Prostate(74;0.00234)|Colorectal(97;0.00629)|Ovarian(174;0.00832)|Breast(144;0.00996)|all_hematologic(6;0.0344)|Hepatocellular(6;0.0742)|Esophageal squamous(6;0.0954) Epithelial(2;2.6e-55)|all cancers(2;2.66e-49)|OV - Ovarian serous cystadenocarcinoma(10;1.48e-39)|Colorectal(2;8.29e-08)|Lung(2;4.47e-07)|STAD - Stomach adenocarcinoma(2;1.11e-05)|COAD - Colon adenocarcinoma(2;0.00012)|LUSC - Lung squamous cell carcinoma(2;0.000775)|LUAD - Lung adenocarcinoma(3;0.0173)|READ - Rectum adenocarcinoma(2;0.0276) Adenosine monophosphate(DB00131)|Dyphylline(DB00651) GGTCATAATCGCTGTCGGATC 0.493000 4 52 0 0 1 0 0 DNAJC11 55735 broad.mit.edu 37 1 6696243 6696243 + Missense_Mutation SNP G G C TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr1:6696243G>C uc001aof.2 - 14 1694 c.1588C>G c.(1588-1590)Cag>Gag p.Q530E DNAJC11_uc001aog.2_Missense_Mutation_p.Q478E|DNAJC11_uc010nzu.1_Missense_Mutation_p.Q440E NM_018198 NP_060668 Q9NVH1 DJC11_HUMAN Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 11 (DNAJC11), mRNA. 530 protein folding heat shock protein binding|unfolded protein binding NS(1)|breast(2)|endometrium(4)|kidney(2)|large_intestine(11)|lung(8)|ovary(2)|skin(1)|urinary_tract(1) 32 Ovarian(185;0.0265)|all_lung(157;0.154) all_cancers(23;1.97e-27)|all_epithelial(116;1.76e-17)|all_lung(118;2.27e-05)|Lung NSC(185;9.97e-05)|Renal(390;0.00188)|Breast(487;0.00289)|Colorectal(325;0.00342)|Hepatocellular(190;0.0218)|Myeloproliferative disorder(586;0.0393)|Ovarian(437;0.156) Colorectal(212;2.34e-07)|COAD - Colon adenocarcinoma(227;2.05e-05)|Kidney(185;7.67e-05)|BRCA - Breast invasive adenocarcinoma(304;0.000639)|KIRC - Kidney renal clear cell carcinoma(229;0.00128)|STAD - Stomach adenocarcinoma(132;0.00179)|READ - Rectum adenocarcinoma(331;0.0649) CCCCGGAACTGATAGAGCACT 0.542000 17 35 0 0 1 0 0 NRAS 4893 broad.mit.edu 37 1 115256529 115256529 + Missense_Mutation SNP T T C rs11554290 TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr1:115256529T>C uc009wgu.3 - 2 436 c.182A>G c.(181-183)cAa>cGa p.Q61R NM_002524 NP_002515 P01111 RASN_HUMAN Homo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), mRNA. 61 Q -> K (in neuroblastoma cell).|Q -> R (in lung carcinoma cell and melanoma; dbSNP:rs11554290). Ras protein signal transduction|activation of MAPKK activity|axon guidance|blood coagulation|epidermal growth factor receptor signaling pathway|insulin receptor signaling pathway|leukocyte migration|nerve growth factor receptor signaling pathway Golgi membrane|plasma membrane GTP binding|GTPase activity p.Q61R(1713)|p.Q61K(635)|p.Q61L(370)|p.Q61H(123)|p.Q61P(46)|p.Q61E(9)|p.G60E(6)|p.Q61?(5)|p.Q61Q(3)|p.G60R(1)|p.G60>?(1)|p.Q61*(1)|p.Q61_E62>HK(1) NS(134)|adrenal_gland(9)|autonomic_ganglia(9)|biliary_tract(6)|bone(1)|breast(9)|central_nervous_system(10)|cervix(2)|endometrium(11)|eye(3)|haematopoietic_and_lymphoid_tissue(1115)|kidney(3)|large_intestine(105)|liver(10)|lung(42)|meninges(2)|ovary(7)|pancreas(5)|prostate(8)|skin(1144)|soft_tissue(37)|stomach(5)|testis(8)|thyroid(359)|upper_aerodigestive_tract(28)|urinary_tract(13) 3085 all_epithelial(7;5.11e-05)|all_lung(7;0.000179)|Lung NSC(6;0.00195)|Lung SC(450;0.211) all_cancers(81;4.64e-07)|all_epithelial(167;4.2e-07)|all_lung(203;9.97e-06)|Lung NSC(69;1.74e-05) Lung(183;0.0234)|Colorectal(144;0.0686)|COAD - Colon adenocarcinoma(174;0.111)|all cancers(265;0.112)|Epithelial(280;0.124)|LUSC - Lung squamous cell carcinoma(189;0.133) GTACTCTTCTTGTCCAGCTGT 0.458000 Q61L(C3A_LIVER)|Q61L(HEPG2_LIVER)|Q61L(HL60_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE)|Q61L(IPC298_SKIN)|Q61L(KMS21BM_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE)|Q61L(MELJUSO_SKIN)|Q61L(MHHES1_BONE)|Q61L(MOLP8_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE)|Q61L(OCIAML3_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE)|Q61P(TF1_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE)|Q61R(HT1197_URINARY_TRACT)|Q61R(KMS27_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE)|Q61R(KU1919_URINARY_TRACT)|Q61R(NCIH2347_LUNG)|Q61R(ONS76_CENTRAL_NERVOUS_SYSTEM)|Q61R(SKMEL2_SKIN)|Q61R(SW1271_LUNG)|Q61R(TT2609C02_THYROID) 50 Mis """melanoma, MM, AML, thyroid""" Noonan syndrome TSP Lung(23;0.16)|Multiple Myeloma(1;<1E-6) 67 103 0 0 1 0 0 CHEK2 11200 broad.mit.edu 37 22 29090097 29090097 + Missense_Mutation SNP G G T TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr22:29090097G>T uc003adt.1 - 13 1585 c.1513C>A c.(1513-1515)Ctt>Att p.L505I CHEK2_uc010gvj.1_Intron|CHEK2_uc003adr.1_Non-coding_Transcript|CHEK2_uc010gvk.1_Non-coding_Transcript|CHEK2_uc003ads.1_Missense_Mutation_p.L241I|CHEK2_uc010gvh.1_Missense_Mutation_p.L371I|CHEK2_uc010gvi.1_Intron|CHEK2_uc003adu.1_Missense_Mutation_p.L462I|CHEK2_uc003adv.1_Missense_Mutation_p.L433I|CHEK2_uc003adx.1_Missense_Mutation_p.L241I NM_001005735 NP_001005735 O96017 CHK2_HUMAN Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 3, mRNA. 462 DNA damage checkpoint|DNA damage response, signal transduction resulting in induction of apoptosis|cell cycle|replicative senescence PML body ATP binding|metal ion binding|protein binding|protein serine/threonine kinase activity central_nervous_system(17)|endometrium(2)|kidney(11)|large_intestine(2)|lung(11)|ovary(1)|prostate(4)|stomach(2) 50 TTCTTGACAAGGTCCAGAGCT 0.448000 F breast Direct reversal of damage;Other conserved DNA damage response genes 6 127 0 0 1 0 0 USP5 8078 broad.mit.edu 37 12 6964920 6964920 + Splice_Site SNP A A G TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr12:6964920A>G uc001qri.4 + 3 297 c.238_splice c.e3-1 p.K80_splice USP5_uc001qrh.4_Splice_Site_p.K80_splice NM_001098536 NP_001092006 P45974 UBP5_HUMAN Homo sapiens ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, mRNA. 80 positive regulation of proteasomal ubiquitin-dependent protein catabolic process|protein K48-linked deubiquitination|ubiquitin-dependent protein catabolic process lysosome cysteine-type endopeptidase activity|omega peptidase activity|protein binding|ubiquitin thiolesterase activity|zinc ion binding breast(6)|endometrium(4)|kidney(2)|large_intestine(6)|lung(14)|skin(2)|urinary_tract(2) 36 TCTTTACAGAAAGAGGAGGAC 0.577000 37 48 0 0 1 0 0 DNAH9 1770 broad.mit.edu 37 17 11827171 11827171 + Missense_Mutation SNP C C A TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr17:11827171C>A uc002gne.3 + 61 11858 c.11790C>A c.(11788-11790)caC>caA p.H3930Q DNAH9_uc010coo.3_Missense_Mutation_p.H3224Q|DNAH9_uc002gnf.3_Missense_Mutation_p.H242Q|DNAH9_uc010vvh.1_Missense_Mutation_p.H283Q NM_001372 NP_001363 Q9NYC9 DYH9_HUMAN Homo sapiens dynein, axonemal, heavy chain 9 (DNAH9), transcript variant 2, mRNA. 3930 AAA 6 (By similarity). cell projection organization|cellular component movement|microtubule-based movement|spermatogenesis axonemal dynein complex|cilium axoneme|cytoplasm|microtubule ATP binding|ATPase activity|microtubule motor activity NS(2)|autonomic_ganglia(1)|breast(15)|central_nervous_system(5)|endometrium(17)|haematopoietic_and_lymphoid_tissue(6)|kidney(17)|large_intestine(49)|liver(2)|lung(123)|ovary(6)|pancreas(1)|prostate(6)|skin(21)|stomach(2)|upper_aerodigestive_tract(13)|urinary_tract(4) 290 Breast(5;0.0122)|all_epithelial(5;0.131) Colorectal(4;6.88e-05)|COAD - Colon adenocarcinoma(4;0.000813)|READ - Rectum adenocarcinoma(10;0.157) AGAACTTTCACAACGTGTCTT 0.453000 6 61 0 0 1 0 0 ZNF703 80139 broad.mit.edu 37 8 37555481 37555481 + Frame_Shift_Del DEL G G - TCGA-EL-A4K2-01A-11D-A257-08 TCGA-EL-A4K2-11A-11D-A25A-08 Untested Somatic Phase_I WXS none Illumina GAIIx ba57094e-ee91-4235-9cfb-88659af9a99e 406c2a3b-2a90-403c-b247-e484b0b313e3 g.chr8:37555481delG uc003xjy.1 + 1 1259 c.1062delG c.(1060-1062)ccgfs p.P354fs NM_025069 NP_079345 Q9H7S9 ZN703_HUMAN Homo sapiens zinc finger protein 703 (ZNF703), mRNA. 354 Gly-rich. adherens junction assembly|mammary gland epithelial cell differentiation|negative regulation of homotypic cell-cell adhesion|negative regulation of transcription, DNA-dependent|positive regulation of cell migration|positive regulation of epithelial to mesenchymal transition|positive regulation of mammary gland epithelial cell proliferation|regulation of canonical Wnt receptor signaling pathway|regulation of cell cycle|regulation of transforming growth factor beta receptor signaling pathway|transcription, DNA-dependent cytoplasm|nucleus nucleic acid binding|protein binding|zinc ion binding FGFR1/ZNF703(2) breast(1)|cervix(1)|kidney(1)|large_intestine(2)|lung(1)|pancreas(1) 7 BRCA - Breast invasive adenocarcinoma(5;7.93e-25)|LUSC - Lung squamous cell carcinoma(8;1.05e-09) GCCTGCCGCCGGGCAAGCCCC 0.711 2 4 --- --- --- ---