Hugo_Symbol	Entrez_Gene_Id	Center	NCBI_Build	Chromosome	Start_Position	End_Position	Strand	Variant_Classification	Variant_Type	Reference_Allele	Tumor_Seq_Allele1	Tumor_Seq_Allele2	dbSNP_RS	dbSNP_Val_Status	Tumor_Sample_Barcode	Matched_Norm_Sample_Barcode	Match_Norm_Seq_Allele1	Match_Norm_Seq_Allele2	Tumor_Validation_Allele1	Tumor_Validation_Allele2	Match_Norm_Validation_Allele1	Match_Norm_Validation_Allele2	Verification_Status	Validation_Status	Mutation_Status	Sequencing_Phase	Sequence_Source	Validation_Method	Score	BAM_File	Sequencer	Tumor_Sample_UUID	Matched_Norm_Sample_UUID	chromosome_name	start	stop	reference	variant	type	gene_name	transcript_name	transcript_species	transcript_source	transcript_version	strand	transcript_status	trv_type	c_position	amino_acid_change	ucsc_cons	domain	all_domains	deletion_substructures	transcript_error	default_gene_name	gene_name_source	 ensembl_gene_id	tier	dbSNP	GMAF	n_ref_count	n_alt_count	normal_vaf	t_ref_count	t_alt_count	tumor_vaf	n_ref_count_val	n_alt_count_val	normal_vaf_val	t_ref_count_val	t_alt_count_val	tumor_vaf_val
MYO3A	0	genome.wustl.edu	37	10	26310463	26310463	+	Missense_Mutation	SNP	C	C	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	T	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	10	26310463	26310463	C	T	SNP	MYO3A	ENST00000265944	human	ensembl	74_37	+1	known	missense	c.617	p.T206I	1.000	pfam_Prot_kinase_dom,pfam_Ser-Thr/Tyr_kinase_cat_dom,superfamily_Kinase-like_dom,smart_Ser/Thr_dual-sp_kinase_dom,smart_Tyr_kinase_cat_dom,pfscan_Prot_kinase_dom	pfam_Myosin_head_motor_dom,pfam_Prot_kinase_dom,pfam_Ser-Thr/Tyr_kinase_cat_dom,pfam_IQ_motif_EF-hand-BS,superfamily_P-loop_NTPase,superfamily_Kinase-like_dom,smart_Ser/Thr_dual-sp_kinase_dom,smart_Tyr_kinase_cat_dom,smart_Myosin_head_motor_dom,smart_IQ_motif_EF-hand-BS,pfscan_IQ_motif_EF-hand-BS,pfscan_Prot_kinase_dom,prints_Myosin_head_motor_dom	-	no_errors	MYO3A	HGNC	ENSG00000095777	tier1	-	-	43	0	0.00	18	32	64.00	136	0	0.00	91	51	35.92
DH3	0	genome.wustl.edu	37	16	21108691	21108691	+	Missense_Mutation	SNP	C	C	G			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	G	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	16	21108691	21108691	C	G	SNP	DH3	ENST00000261383	human	ensembl	74_37	-1	known	missense	c.2650	p.E884Q	0.999	pfam_Dynein_heavy_dom-2,superfamily_P-loop_NTPase	pfam_Dynein_heavy_dom,pfam_Dynein_heavy_dom-2,pfam_ATPase_dyneun-rel_AAA,superfamily_P-loop_NTPase,superfamily_Prefoldin,smart_AAA+_ATPase	-	no_errors	DH3	HGNC	ENSG00000158486	tier1	-	-	37	0	0.00	24	14	36.84	97	0	0.00	57	34	37.36
RASAL2	0	genome.wustl.edu	37	1	178063820	178063820	+	Missense_Mutation	SNP	C	C	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	T	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	1	178063820	178063820	C	T	SNP	RASAL2	ENST00000367649	human	ensembl	74_37	+1	known	missense	c.193	p.R65W	1.000	smart_Pleckstrin_homology	pfam_DUF3498,pfam_RasGAP,pfam_Pleckstrin_homology,pfam_C2_dom,superfamily_Rho_GTPase_activation_prot,superfamily_C2_dom,superfamily_PP1_inhibitor,smart_Pleckstrin_homology,smart_C2_dom,smart_RasGAP,pfscan_Pleckstrin_homology,pfscan_RasGAP	-	no_errors	RASAL2	HGNC	ENSG00000075391	tier1	-	-	34	0	0.00	13	13	50.00	72	0	0.00	60	41	40.59
TMEM132D	0	genome.wustl.edu	37	12	129694177	129694177	+	Missense_Mutation	SNP	G	G	A			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G	G	A	G	G	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	12	129694177	129694177	G	A	SNP	TMEM132D	ENST00000422113	human	ensembl	74_37	-1	known	missense	c.1331	p.T444M	1.000	NULL	NULL	-	no_errors	TMEM132D	HGNC	ENSG00000151952	tier1	-	-	49	0	0.00	26	17	39.53	59	0	0.00	42	24	36.36
GSTA4	0	genome.wustl.edu	37	6	52849261	52849261	+	Splice_Site	SNP	C	C	A			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	A	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	6	52849261	52849261	C	A	SNP	GSTA4	ENST00000370959	human	ensembl	74_37	-1	known	splice_site	c.414+1	e4+1	1.000	-	-	-	no_errors	GSTA4	HGNC	ENSG00000170899	tier1	-	-	208	0	0.00	20	90	81.82	173	0	0.00	27	99	78.57
F5	0	genome.wustl.edu	37	1	169510957	169510957	+	Missense_Mutation	SNP	T	T	C			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	T	T	T	C	T	T	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	1	169510957	169510957	T	C	SNP	F5	ENST00000367797	human	ensembl	74_37	-1	known	missense	c.3371	p.E1124G	0.277	NULL	pfam_Coagulation_fac_5/8-C_type_dom,pfam_Cu-oxidase_3,superfamily_Galactose-bd-like,superfamily_Cupredoxin,smart_Coagulation_fac_5/8-C_type_dom,pfscan_Coagulation_fac_5/8-C_type_dom	-	no_errors	F5	HGNC	ENSG00000198734	tier1	-	-	105	0	0.00	65	44	40.37	124	0	0.00	86	37	30.08
SPPL2C	0	genome.wustl.edu	37	17	43924079	43924079	+	Missense_Mutation	SNP	G	G	A			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G	G	A	G	G	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	17	43924079	43924079	G	A	SNP	SPPL2C	ENST00000329196	human	ensembl	74_37	+1	known	missense	c.1807	p.G603S	1.000	NULL	pfam_Peptidase_A22B_SPP,pfam_Protease-assoc_domain,smart_Preselin/SPP	-	no_errors	SPPL2C	HGNC	ENSG00000185294	tier1	-	-	32	0	0.00	21	7	25.00	71	0	0.00	66	31	31.63
C10orf68	0	genome.wustl.edu	37	10	33140795	33140795	+	Missense_Mutation	SNP	C	C	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	T	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	10	33140795	33140795	C	T	SNP	C10orf68	ENST00000375025	human	ensembl	74_37	+1	known	missense	c.1877	p.T626I	0.000	NULL	NULL	-	no_errors	C10orf68	HGNC	ENSG00000150076	tier1	-	-	43	0	0.00	44	6	12.00	154	1	0.64	132	23	14.84
FAM134A	0	genome.wustl.edu	37	2	220045810	220045810	+	Missense_Mutation	SNP	G	G	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G	G	T	G	G	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	2	220045810	220045810	G	T	SNP	FAM134A	ENST00000430297	human	ensembl	74_37	+1	known	missense	c.667	p.V223L	1.000	pfam_Reticulon	pfam_Reticulon	-	no_errors	FAM134A	HGNC	ENSG00000144567	tier1	-	-	85	0	0.00	38	30	44.12	124	0	0.00	74	50	40.32
MIR200A	0	genome.wustl.edu	37	1	1103249	1103249	+	R	SNP	C	C	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	T	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	1	1103249	1103249	C	T	SNP	MIR200A	ENST00000384875	human	ensembl	74_37	+1	known	rna	NULL	NULL	1.000	-	-	-	no_errors	MIR200A	HGNC	ENSG00000207607	tier1	-	-	111	0	0.00	9	60	86.96	44	0	0.00	2	20	90.91
TP53	0	genome.wustl.edu	37	17	7578406	7578406	+	Missense_Mutation	SNP	C	C	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	C	C	C	T	C	C	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	17	7578406	7578406	C	T	SNP	TP53	ENST00000269305	human	ensembl	74_37	-1	known	missense	c.524	p.R175H	1.000	pfam_p53_D-bd,superfamily_p53-like_TF_D-bd,prints_p53_tumour_suppressor	pfam_p53_D-bd,pfam_p53_tetrameristn,pfam_p53_transactivation_domain,superfamily_p53-like_TF_D-bd,superfamily_p53_tetrameristn,prints_p53_tumour_suppressor	-	no_errors	TP53	HGNC	ENSG00000141510	tier1	rs28934578	-	30	0	0.00	2	17	89.47	47	0	0.00	8	31	79.49
FAM196B	0	genome.wustl.edu	37	5	169309787	169309787	+	Silent	SNP	A	A	G			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	A	A	A	G	A	A	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	5	169309787	169309787	A	G	SNP	FAM196B	ENST00000377365	human	ensembl	74_37	-1	known	silent	c.1116	p.N372	0.000	NULL	NULL	-	no_errors	FAM196B	HGNC	ENSG00000204767	tier1	-	-	55	0	0.00	61	7	10.29	70	0	0.00	79	7	8.14
SEH1L	0	genome.wustl.edu	37	18	12986927	12986929	+	In_Frame_Del	DEL	TCC	TCC	-			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	TCC	TCC	TCC	-	TCC	TCC	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	18	12986927	12986929	TCC	-	DEL	SEH1L	ENST00000399892	human	ensembl	74_37	+1	known	in_frame_del	c.1137_1139	p.P383in_frame_del	0.997:1.000:1.000	NULL	pfam_WD40_repeat,superfamily_WD40_repeat_dom,smart_WD40_repeat,pfscan_WD40_repeat,pfscan_WD40_repeat_dom,prints_G-protein_beta_WD-40_rep	-	no_errors	SEH1L	HGNC	ENSG00000085415	tier1			25	0	0.00	25	4	13.79	59	0	0.00	50	4	7.41
TMED10	0	genome.wustl.edu	37	14	75601711	75601712	+	Splice_Site	INS	-	-	A			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	-	-	-	A	-	-	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	14	75601711	75601712	-	A	INS	TMED10	ENST00000303575	human	ensembl	74_37	-1	known	splice_site_ins	c.539-3_539-2	e5-2	1.000:0.961	-	-	-	no_errors	TMED10	HGNC	ENSG00000170348	tier1			38	0	0.00	27	3	10.00	70	1	1.41	80	4	4.76
TTLL5	0	genome.wustl.edu	37	14	76420882	76420882	+	R	DEL	T	T	-			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	T	T	T	-	T	T	Unknown	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	14	76420882	76420882	T	-	DEL	TTLL5	ENST00000554972	human	ensembl	74_37	+1	known	rna	NULL	NULL	0.005	-	-	-	no_errors	TTLL5	HGNC	ENSG00000119685	tier1			25	0	0.00	33	4	10.81	45	1	2.17	55	11	16.67
HLA-DRB1	0	genome.wustl.edu	37	6	32548628	32548628	+	Missense_Mutation	SNP	G	G	A			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G					Unknown	Untested	Somatic	Phase_IV	WXS	none			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	6	32548628	32548628	G	A	SNP	HLA-DRB1	ENST00000360004	human	ensembl	74_37	-1	known	missense	c.658	p.R220W	0.988	NULL	pfam_MHC_II_b_N,pfam_Ig_C1-set,superfamily_MHC_I/II-like_Ag-recog,smart_MHC_II_b_N,smart_Ig_C1-set,pfscan_Ig-like_dom	-	no_errors	HLA-DRB1	HGNC	ENSG00000196126	tier1	rs34624872	-	133	1	0.75	35	9	20.45	49	0	0.00	9	0	0.00
CES1P1	0	genome.wustl.edu	37	16	55803899	55803899	+	R	SNP	G	G	A			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G					Unknown	Untested	Somatic	Phase_IV	WXS	none			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	16	55803899	55803899	G	A	SNP	CES1P1	ENST00000571348	human	ensembl	74_37	+1	known	rna	NULL	NULL	0.860	-	-	-	no_errors	CES1P1	HGNC	ENSG00000228695	tier1	-	-	14	0	0.00	11	4	26.67	0	0	0.00	0	0	0.00
ENSG00000235704	0	genome.wustl.edu	37	22	20378616	20378628	+	R	DEL	GGGATCCGGGGGT	GGGATCCGGGGGT	-			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	GGGATCCGGGGGT	GGGATCCGGGGGT					Unknown	Untested	Somatic	Phase_IV	WXS	none			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	22	20378616	20378628	GGGATCCGGGGGT	-	DEL	ENSG00000235704	ENST00000426653	human	ensembl	74_37	+1	known	rna	NULL	NULL	0.009:0.014:0.006:0.004:0.011:0.028:0.217:0.219:0.178:0.177:0.193:0.194:0.213	-	-	-	no_errors	AC023490.2	Clone_based_vega_gene	ENSG00000235704	tier1			0	0	0.00	0	0	0.00	0	0	0.00	0	0	0.00
HES3	0	genome.wustl.edu	37	1	6305511	6305511	+	Missense_Mutation	SNP	G	G	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G					Unknown	Untested	Somatic	Phase_IV	WXS	none			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	1	6305511	6305511	G	T	SNP	HES3	ENST00000377898	human	ensembl	74_37	+1	known	missense	c.505	p.G169W	0.007	NULL	pfam_bHLH_dom,superfamily_bHLH_dom,smart_bHLH_dom,pfscan_bHLH_dom	-	no_errors	HES3	HGNC	ENSG00000173673	tier1	-	-	80	0	0.00	29	4	12.12	3	0	0.00	2	0	0.00
SDHAP1	0	genome.wustl.edu	37	3	195687221	195687221	+	R	SNP	A	A	G			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	A	A					Unknown	Untested	Somatic	Phase_IV	WXS	none			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	3	195687221	195687221	A	G	SNP	SDHAP1	ENST00000427149	human	ensembl	74_37	-1	known	rna	NULL	NULL	0.009	-	-	-	no_errors	SDHAP1	HGNC	ENSG00000185485	tier1	rs62282781	-	14	0	0.00	8	5	38.46	3	0	0.00	6	0	0.00
TELO2	0	genome.wustl.edu	37	16	1550652	1550652	+	Missense_Mutation	SNP	G	G	T			TCGA-DX-A48J-01A-21D-A307-09	TCGA-DX-A48J-10A-01D-A307-09	G	G					Unknown	Untested	Somatic	Phase_IV	WXS	none			Illumina HiSeq	c31d841a-1b54-4e64-bde0-e458668a7f88	130d56bf-7533-4fbc-bea1-bc8973a3b123	16	1550652	1550652	G	T	SNP	TELO2	ENST00000262319	human	ensembl	74_37	+1	known	missense	c.1233	p.E411D	0.755	NULL	pfam_Telomere_length_regulation_dom,superfamily_ARM-type_fold	-	no_errors	TELO2	HGNC	ENSG00000100726	tier1	-	-	32	0	0.00	22	4	15.38	25	0	0.00	34	2	5.56