Hugo_Symbol	Entrez_Gene_Id	Center	NCBI_Build	Chromosome	Start_Position	End_Position	Strand	Variant_Classification	Variant_Type	Reference_Allele	Tumor_Seq_Allele1	Tumor_Seq_Allele2	dbSNP_RS	dbSNP_Val_Status	Tumor_Sample_Barcode	Matched_Norm_Sample_Barcode	Match_Norm_Seq_Allele1	Match_Norm_Seq_Allele2	Tumor_Validation_Allele1	Tumor_Validation_Allele2	Match_Norm_Validation_Allele1	Match_Norm_Validation_Allele2	Verification_Status	Validation_Status	Mutation_Status	Sequencing_Phase	Sequence_Source	Validation_Method	Score	BAM_File	Sequencer	Tumor_Sample_UUID	Matched_Norm_Sample_UUID	chromosome_name	start	stop	reference	variant	type	gene_name	transcript_name	transcript_species	transcript_source	transcript_version	strand	transcript_status	trv_type	c_position	amino_acid_change	ucsc_cons	domain	all_domains	deletion_substructures	transcript_error	NormalRefReads_WU	NormalVarReads_WU	NormalVAF_WU	TumorRefReads_WU	TumorVarReads_WU	TumorVAF_WU	RNARefReads_WU	RNAVarReads_WU	RNAVAF_WU
ATG4C	0	genome.wustl.edu	36	1	63073082	63073082	+	Missense_Mutation	SNP	G	G	T			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	G	G	G	T	G	G	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	1	63073082	63073082	G	T	SNP	ATG4C	NM_032852.2	human	genbank	54_36p	+1	reviewed	missense	c.1060	p.D354Y	1.000	HMMPfam_Peptidase_C54	HMMPfam_Peptidase_C54	-	no_errors	86	7	7.53	308	125	28.67	21	9	30.00
NRAS	0	genome.wustl.edu	36	1	115058053	115058053	+	Missense_Mutation	SNP	G	G	T	rs121913254		TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	G	G	G	T	G	G	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	1	115058053	115058053	G	T	SNP	NRAS	NM_002524.3	human	genbank	54_36p	-1	reviewed	missense	c.181	p.Q61K	1.000	HMMSmart_SM00173,HMMSmart_SM00174,HMMSmart_SM00175,HMMPfam_Ras,superfamily_P-loop containing nucleoside triphosphate hydrolases	HMMSmart_SM00173,HMMSmart_SM00174,HMMSmart_SM00175,HMMPfam_Ras,superfamily_P-loop containing nucleoside triphosphate hydrolases	-	no_errors	73	3	3.95	245	55	18.21	96	16	14.29
SEMA4A	0	genome.wustl.edu	36	1	154412904	154412904	+	Missense_Mutation	SNP	A	A	G			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	A	A	A	G	A	A	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	1	154412904	154412904	A	G	SNP	SEMA4A	NM_022367.2	human	genbank	54_36p	+1	provisional	missense	c.1778	p.H593R	0.437	NULL	HMMPfam_Sema,HMMSmart_Sema,superfamily_Sema,HMMPfam_PSI,HMMSmart_PSI,superfamily_Plexin-like_fold	-	no_errors	229	31	11.92	348	314	47.08	58	43	42.16
SPEG	0	genome.wustl.edu	36	2	220052132	220052132	+	Nonsense_Mutation	SNP	C	C	T			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	C	C	C	T	C	C	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	2	220052132	220052132	C	T	SNP	SPEG	NM_005876.4	human	genbank	54_36p	+1	validated	nonsense	c.5050	p.R1684*	0.643	HMMSmart_SM00220,superfamily_Protein kinase-like (PK-like),HMMPfam_Pkinase	HMMSmart_SM00219,HMMSmart_SM00220,HMMSmart_SM00408,HMMSmart_SM00409,HMMPfam_fn3,HMMSmart_SM00060,PatternScan_PROTEIN_KINASE_ST,superfamily_Fibronectin type III,superfamily_Protein kinase-like (PK-like),HMMPfam_I-set,PatternScan_PROTEIN_KINASE_ATP,HMMPfam_Pkinase,superfamily_Immunoglobulin	-	no_errors	79	11	12.22	88	66	42.31	NA	NA	NA
COL12A1	0	genome.wustl.edu	36	6	75958768	75958768	+	Missense_Mutation	SNP	G	G	A			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	G	G	G	A	G	G	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	6	75958768	75958768	G	A	SNP	COL12A1	NM_004370.5	human	genbank	54_36p	-1	reviewed	missense	c.214	p.L72F	1.000	HMMPfam_fn3,HMMSmart_SM00060,superfamily_Fibronectin type III	HMMPfam_VWA,HMMSmart_SM00327,HMMSmart_SM00210,HMMPfam_fn3,HMMSmart_SM00060,HMMPfam_Collagen,superfamily_Fibronectin type III,superfamily_Concanavalin A-like lectins/glucanases,superfamily_vWA-like	-	no_errors	29	2	6.45	153	104	40.00	NA	NA	NA
OR10W1	0	genome.wustl.edu	36	11	57791808	57791808	+	Silent	SNP	G	G	A			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	G	G	G	A	G	G	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	11	57791808	57791808	G	A	SNP	OR10W1	NM_207374.2	human	genbank	54_36p	-1	provisional	silent	c.99	p.N33	0.032	HMMPfam_7tm_1,superfamily_SSF81321	HMMPfam_7tm_1,PatternScan_G_PROTEIN_RECEP_F1_1,superfamily_SSF81321	-	no_errors	90	13	12.62	262	219	44.97	NA	NA	NA
POLR3B	0	genome.wustl.edu	36	12	105294314	105294314	+	Missense_Mutation	SNP	C	C	T			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	C	C	C	T	C	C	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	12	105294314	105294314	C	T	SNP	POLR3B	NM_018082.4	human	genbank	54_36p	+1	provisional	missense	c.452	p.T151M	0.998	HMMPfam_RNA_pol_Rpb2_1,superfamily_beta and beta-prime subunits of DNA dependent RNA-polymerase	HMMPfam_RNA_pol_Rpb2_6,PatternScan_RNA_POL_BETA,HMMPfam_RNA_pol_Rpb2_7,HMMPfam_RNA_pol_Rpb2_2,HMMPfam_RNA_pol_Rpb2_1,HMMPfam_RNA_pol_Rpb2_3,HMMPfam_RNA_pol_Rpb2_4,HMMPfam_RNA_pol_Rpb2_5,superfamily_beta and beta-prime subunits of DNA dependent RNA-polymerase	-	no_errors	96	6	5.88	188	131	40.31	3	7	70.00
FLT3	0	genome.wustl.edu	36	13	27506258	27506275	+	In_Frame_Ins	DEL	CATATTCATATTCTCTGA	CATATTCATATTCTCTGA	GGGGTGGGGGGG			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	CATATTCATATTCTCTGA	CATATTCATATTCTCTGA	CATATTCATATTCTCTGA	GGGGTGGGGGGG	CATATTCATATTCTCTGA	CATATTCATATTCTCTGA	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	13	27506258	27506275	CATATTCATATTCTCTGA	GGGGTGGGGGGG	INS	FLT3	NM_004119.2	human	genbank	54_36p	-1	reviewed	in_frame_ins	c.1798_1781	p.595in_frame_insPPTP	1.000:1.000:1.000:0.999:0.789:0.821:0.816:0.676:0.765:0.789:0.794:0.991:0.997:0.996:0.999:1.000:1.000:1.000	superfamily_Kinase_like	HMMPfam_Pkinase_Tyr,HMMSmart_TyrKc,PatternScan_RECEPTOR_TYR_KIN_III,PatternScan_PROTEIN_KINASE_TYR,superfamily_Kinase_like,HMMPfam_ig,PatternScan_PROTEIN_KINASE_ATP,superfamily_SSF48726	-	no_errors	NA	NA	NA	NA	NA	NA	NA	NA	NA
HCN4	0	genome.wustl.edu	36	15	71411572	71411572	+	Silent	SNP	G	G	A			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	G	G	G	A	G	G	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	15	71411572	71411572	G	A	SNP	HCN4	NM_005477.2	human	genbank	54_36p	-1	reviewed	silent	c.1324	p.L442	1.000	HMMPfam_Ion_trans,superfamily_SSF81324	HMMPfam_cNMP_binding,HMMSmart_cNMP,HMMPfam_Ion_trans,HMMPfam_Ion_trans_N,PatternScan_CNMP_BINDING_1,PatternScan_CNMP_BINDING_2,superfamily_cNMP_binding,superfamily_SSF81324	-	no_errors	28	1	3.45	77	64	45.07	NA	NA	NA
NAGPA	0	genome.wustl.edu	36	16	5018917	5018917	+	Silent	SNP	G	G	C			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	G	G	G	C	G	G	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	16	5018917	5018917	G	C	SNP	NAGPA	NM_016256.2	human	genbank	54_36p	-1	reviewed	silent	c.885	p.L295	0.994	NULL	HMMSmart_EGF,PatternScan_EGF_1,PatternScan_EGF_2,HMMPfam_EGF_2	-	no_errors	59	6	9.09	75	82	51.25	21	27	56.25
COL5A3	0	genome.wustl.edu	36	19	9973274	9973274	+	Missense_Mutation	SNP	C	C	T			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	C	C	C	T	C	C	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	19	9973274	9973274	C	T	SNP	COL5A3	NM_015719.3	human	genbank	54_36p	-1	reviewed	missense	c.1036	p.G346R	0.014	NULL	HMMPfam_COLFI,HMMSmart_SM00038,HMMSmart_SM00210,HMMPfam_Collagen,superfamily_Concanavalin A-like lectins/glucanases,HMMPfam_Laminin_G_2	-	no_errors	117	7	5.65	205	138	40.00	NA	NA	NA
CDK13	0	genome.wustl.edu	36	7	40084184	40084184	+	Frame_Shift_Del	DEL	A	A	-			TCGA-AB-2870-03A-01W-0732-08	TCGA-AB-2870-11A-01W-0732-08	A	A	A	-	A	A	Verified	Valid	Somatic	Phase_IV	WXS	Hybrid_Capture_Illumina_Seq	1	dbGAP	Illumina HiSeq	9ad67fd3-5750-42ab-b4e2-55133613a3e4	ab21f718-4b02-4b59-9590-a0de74285788	7	40084184	40084184	A	-	DEL	CDC2L5	NM_003718.3	human	genbank	54_36p	+1	reviewed	frame_shift_del	c.2836	p.Y946fs	0.975	HMMSmart_SM00219,HMMSmart_SM00220,superfamily_Protein kinase-like (PK-like),HMMPfam_Pkinase	HMMSmart_SM00219,HMMSmart_SM00220,PatternScan_PROTEIN_KINASE_ST,superfamily_Protein kinase-like (PK-like),PatternScan_PROTEIN_KINASE_ATP,HMMPfam_Pkinase	-	no_errors	1	0	0.00	5	0	0.00	0	0	0.00